Bulk order Acrobiosystems for Chinese

Chinese

A-Z

Siglec-15 | CD20 | FGL1 | CD19 | PD-1 | BCMA

There is no goods in the shopping cart !

My Cart

A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9

Home
Product & Service

Products by Category

Recombinant proteins and more
Immune Checkpoint ProteinsHot Targets of CAR-T Cell TherapyNew Biosimilar Drug TargetsHot Anti-idiotypic AntibodyHot Fc Receptor Proteins Llama IgG Fc Fusion ProteinNew Bio-Markers & CD Antigens ActiveMax -Best Cytokines Viral Proteins Enzymes Antibodies All Recombinant Proteins

Pre-labeled proteins
Biotinylated protein collectionHot FITC & PE labeled ProteinsNew Fluorescent fusion proteins
Kits
ELISA Assay Kits for Quantitative Analysis of Therapeutic Antibodies in Serum SamplesNew Inhibitor Screening Kits Five-Minute MAB Isotyping Kits
Others
HPLC-verified Proteins

Hot Targets

Siglec-15 - Validated by Cell-based Assay BLI/ SPR/ ELISANew Integrin - Integrin ProteinsNew CD20 - Full Length ProteinExclusive FGL1 - Fibrinogen-like protein 1New PD1 - PD1 Pathway TargetsHot CD19 - High Affinity CD19, Designed for Detection of Anti-CD19 CAR ExpressionNew CD3E - Biotinylated CD3E productsHot BCMA - B-Cell Maturation Antigen TIGIT - TIGIT Signaling Axis CD47 - Leukocyte Surface Antigen CD47 LAG3 - Lymphocyte-activation gene 3 ROR - Recombinant ROR1 & ROR2
Services

Innovation Lab ServicesNew
Support

How to Order Shipping & Payments Global Distributors Testimonials Download Brochures Download COA
Application

Quantitative Analysis of mAb in Serum Evaluation of CAR Expression
X-LAB
Company

About Us Join Us Contact Us
Activity

News Exhibitions Promotion

Your Position: Home > Receptor Tyrosine Kinases > VEGF R2 > Mouse VEGF R2 /KDR Protein, Mouse IgG2a Fc Tag, low endotoxin

Mouse VEGF R2 / KDR Protein, Mouse IgG2a Fc Tag, low endotoxin

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
KDR,CD309,FLK1,VEGFR,VEGFR2
Source
Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (VE2-M5258) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Glu 762 (Accession # P35918-1).
Predicted N-terminus: Ala 20
Request for sequence
Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 110.1 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA

Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxinMouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) ELISA bioactivity

Immobilized ActiveMax® Human VEGF165, Tag Free (HPLC-verified) (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) with a linear range of 1-31 ng/mL (QC tested).

Protocol

Immobilized ActiveMax® Mouse VEGF120, Tag Free (Cat. No. VE0-M4211) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) with a linear range of 1-31 ng/mL (Routinely tested).

Protocol

Immobilized ActiveMax® Mouse VEGF164, Tag Free (HPLC-verified) (Cat. No. VE4-M4216) at 2 μg/mL (100 μL/well) can bind Mouse VEGF R2, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. VE2-M5258) with a linear range of 2-31 ng/mL (Routinely tested).

Protocol

Background
Kinase insert domain receptor (KDR) is also known as CD309, FLK1, VEGFR, VEGFR2, and is one of the subtypes of VEGFR. VEGF receptors are receptors for vascular endothelial growth factor (VEGF). There are three main subtypes of VEGFR, numbered 1, 2 and 3. The VEGF receptors have an extracellular portion consisting of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion containing a split tyrosine-kinase domain. VEGF-A binds to VEGFR-1 (Flt-1) and VEGFR-2 (KDR/Flk-1). VEGFR-2 appears to mediate almost all of the known cellular responses to VEGF.The function of VEGFR-1 is less well defined, although it is thought to modulate VEGFR-2 signaling. Another function of VEGFR-1 may be to act as a dummy/decoy receptor, sequestering VEGF from VEGFR-2 binding (this appears to be particularly important during vasculogenesis in the embryo). In addition, VEGFR2 is able to interact with HIV-1 extracellular Tat protein upon VEGF activation, and seems to enhance angiogenesis in Kaposi's sarcoma lesions.
References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.