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Your Position: Home > All Other Proteins > Mesothelin > Human MSLN /Mesothelin (aa 296-580) Protein, Fc Tag

Human Mesothelin / MSLN (296-580) Protein, Fc Tag

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Human Mesothelin (296-580), Fc Tag (MSN-H5253) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
    Predicted N-terminus: Glu 296
  • Molecular Characterization
    Online(Glu 296 - Gly 580) AAH09272.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 58.6 kDa. The protein migrates as 66-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) SDS-PAGE gel

Human Mesothelin (296-580), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human Mesothelin (296-580), Fc TagHuman Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) ELISA bioactivity

Immobilized Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253) at 0.5 μg/mL (100 μL/well) can bind Anti-Human MSLN Mab with a linear range of 0.1-3 ng/mL (QC tested).

Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253, 1 µg/ml) and C. Fc Tag Fusion Protein Control, followed by FITC anti-human IgG Fc. A. Non-transfected 293 cells and C. Fc Tag Fusion Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H5253).

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