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Your Position: Home > Recombinant Proteins > Glypican 3 > Biotinylated Human Glypican 3 / GPC3 Protein, His Tag, Avi Tag (Avitag™)

Biotinylated Human Glypican 3 / GPC3 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Biotinylated Human Glypican 3, His Tag, Avi Tag (GP3-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1).
    Predicted N-terminus: Gln 25, Ser 359
  • Molecular Characterization
    Glypican 3(Gln 25 - His 559) P51654-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 64.5 kDa. The protein migrates as 42 kDa(caused by a furin cleavage site) and 70-250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human Glypican 3, His Tag, Avi Tag (Cat. No. GP3-H82E5) SDS-PAGE gel

Biotinylated Human Glypican 3, His Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity
Biotinylated Human Glypican 3, His Tag, Avi TagBiotinylated Human Glypican 3, His Tag, Avi Tag (Cat. No. GP3-H82E5) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 3, His Tag, Avi Tag (Cat. No. GP3-H82E5) with a linear range of 1-16 ng/mL (QC tested).

Biotinylated Human Glypican 3, His Tag, Avi TagBiotinylated Human Glypican 3, His Tag, Avi Tag (Cat. No. GP3-H82E5) ELISA bioactivity

Immobilized Anti-Human GPC3 Mab at 2 μg/mL (100 μL/well) can bind Biotinylated Human Glypican 3, His Tag, Avi Tag (Cat. No. GP3-H82E5) with a linear range of 0.4-6 ng/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-GPC3 CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-GPC3-scFv and RFP tag. 2e5 of the cells were first stained with B. biotinylated human Glypican 3 / GPC3 protein, His Tag, Avi Tag (Cat. No. GP3-H82E5, 10ug/ml) and C. biotinylated protein control, followed by FITC-conjugated streptavidin. A. Non-transfected 293 cells and C. biotinylated protein control were used as negative control. RFP was used to evaluate CAR (anti-GPC3-scFv) expression and FITC was used to evaluate the binding activity of biotinylated human Glypican 3 / GPC3 protein, His Tag, Avi Tag (Cat. No. GP3-H82E5).

  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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