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Your Position: Home > All Other Proteins > Integrin alpha 2b beta 3 > Human Integrin alpha 2b beta 3 (ITGAIIb&ITGB3) Heterodimer Protein

Human Integrin alpha 2b beta 3 (ITGAIIb&ITGB3) Heterodimer Protein

  • Synonym
    Integrin alpha 2b beta 3,ITGAIIb&ITGB3
  • Source
    Recombinant Human ITGAIIb & ITGB3 Heterodimer Protein (IT3-H52W8) is expressed from human 293 cells (HEK293). It contains AA Leu 32 - Arg 993 (ITGAIIb) & Gly 27 - Asp 718 (ITGB3) (Accession # P08514-1 (ITGAIIb) (R887L) & P05106-1 (ITGB3)).
    Predicted N-terminus: Leu 32 (ITGAIIb) & Gly 27 (ITGB3)
  • Molecular Characterization
    ITGAIIb&ITGB3(Leu 32 - Arg 993 (ITGAIIb) & Gly 27 - Asp 718 (ITGB3)) P08514-1 (R887L) (ITGAIIb) & P05106-1 (ITGB3)

    Human ITGAIIb & ITGB3 Heterodimer Protein, produced by co-expression of ITGAIIb and ITGB3, has a calculated MW of 111.4 kDa (ITGAIIb) & 81.8 kDa (ITGB3). Subunit ITGAIIb is  fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Leu 32 (ITGAIIb) & Gly 27 (ITGB3). The reducing (R) protein migrates as 110-120 kDa, 125 kDa and 145 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human ITGAIIb & ITGB3 (Cat. No. IT3-H52W8) SDS-PAGE gel

Human ITGAIIb & ITGB3 on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human ITGAIIb & ITGB3Human ITGAIIb & ITGB3 (Cat. No. IT3-H52W8) ELISA bioactivity

Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Human ITGAIIb & ITGB3 (Cat. No. IT3-H52W8) with a linear range of 0.16-5 μg/mL (QC tested).

Human ITGAIIb & ITGB3Human ITGAIIb & ITGB3 (Cat. No. IT3-H52W8) ELISA bioactivity

Immobilized Human ITGAIIb & ITGB3 (Cat. No. IT3-H52W8) at 1 μg/mL (100 μL/well) can bind Abciximab with a linear range of 0.8-6 ng/mL (Routinely tested).

  • Background
    Integrin alpha IIb beta 3 exist in a conformational equilibrium clustered around four main states. These conformations range from a compact bent nodule to two partially extended intermediate conformers and finally to a fully upright state. Activation of blood platelets by physiological stimuli at sites of vascular injury induces inside-out signaling, resulting in a conformational change of the prototype Integrin alpha IIb beta 3 from an inactive to an active state competent to bind soluble fibrinogen. Furthermore, ligand occupancy of Integrin alpha IIb beta 3 outside-in signaling and additional conformational changes of the receptor, leading to the exposure of extracellular neoepitopes termed ligand-induced binding sites (LIBS), which are recognized by anti-LIBS monoclonal antibodies.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $410.00

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