>Integrin alpha 2b beta 3
>Human Integrin alpha 2b beta 3 (ITGAIIb&ITGB3) Heterodimer Protein
Human ITGAIIb & ITGB3 Heterodimer Protein, produced by co-expression of ITGAIIb and ITGB3, has a calculated MW of 111.4 kDa (ITGAIIb) & 81.8 kDa (ITGB3). Subunit ITGAIIb is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Leu 32 (ITGAIIb) & Gly 27 (ITGB3). The reducing (R) protein migrates as 110-120 kDa, 125 kDa and 145 kDa due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human ITGAIIb & ITGB3 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Human ITGAIIb & ITGB3 (Cat. No. IT3-H52W8) with a linear range of 0.16-5 μg/mL (QC tested).
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