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Your Position: Home > Immune Checkpoint Proteins > LILRA6 > Biotinylated Human LILRA6 / CD85b / ILT8 Protein, His Tag, Avi Tag (Avitag™)

Biotinylated Human LILRA6 / CD85b / ILT8 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    LILRA6,CD85b,ILT8,ILT-8
  • Source
    Biotinylated Human LILRA6, His Tag, Avi Tag (LI6-H82E2) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Asn 447 (Accession # Q6PI73-1).
    Predicted N-terminus: Gly 24
  • Molecular Characterization
    Online(Gly 24 - Asn 447) Q6PI73-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 50.0 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human LILRA6, His Tag, Avi Tag (Cat. No. LI6-H82E2) SDS-PAGE gel

Biotinylated Human LILRA6, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human LILRA6, His Tag, Avi TagBiotinylated Human LILRA6, His Tag, Avi Tag (Cat. No. LI6-H82E2) ELISA bioactivity

Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LILRA6, His Tag, Avi Tag (Cat. No. LI6-H82E2) with a linear range of 78-625 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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