This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.
The protein has a calculated MW of 18.6 kDa. As a result of glycosylation, the protein migrates as 11 kDa and 18-21 kDa under reducing (R) condition, and 22-28 kDa under non-reducing (NR) condition (SDS-PAGE).
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Biotinylated Human TSLP, His Tag, Avi Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Immobilized Human TSLP R, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His Tag, Avi Tag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).
Immobilized Anti-TSLP, Human IgG2 at 2 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His Tag, Avi Tag (Cat. No. TSP-H82Eb) with a linear range of 0.1-2 ng/mL (Routinely tested).
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Inventory : In stock
Price(USD) : $1380.00
Inventory : In stock
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