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Your Position: Home > Recombinant Proteins > LAG-3 > Marmoset LAG-3 / CD223 Protein

Marmoset LAG-3 / CD223 Protein

  • Synonym
    LAG3,CD223,FDC
  • Source
    Marmoset LAG-3 (LA3-M52Ha) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - His 449 (Accession # F7HXP9-1).
    Predicted N-terminus: Ala 18
  • Molecular Characterization
    LAG-3(Ala 18 - His 449) F7HXP9-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 48.0 kDa. The protein migrates as 57 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Marmoset LAG-3 (Cat. No. LA3-M52Ha) SDS-PAGE gel

Marmoset LAG-3 on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Marmoset LAG-3Marmoset LAG-3 (Cat. No. LA3-M52Ha) ELISA bioactivity

Immobilized Madarex LAG-3 Mab, Human IgG1 at 2 μg/mL (100 μL/well) can bind Marmoset LAG-3 (Cat. No. LA3-M52Ha) with a linear range of 10-156 ng/mL (QC tested).

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