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Your Position: Home > All Other Proteins > BCMA > Biotinylated Human BCMA / TNFRSF17 Protein, His Tag, Avi Tag

Biotinylated Human BCMA / TNFRSF17 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Biotinylated Human BCMA, His Tag, Avi Tag (BCA-H82E4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Online(Met 1 - Ala 54) Q02223-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 9.6 kDa. The protein migrates as 12 kDa and 15-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4) SDS-PAGE gel

Biotinylated Human BCMA, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human BCMA, His Tag, Avi TagBiotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4) ELISA bioactivity

Immobilized Human BAFF, Fc Tag (Cat. No. BAF-H4268) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4) with a linear range of 2-31 ng/mL (QC tested).

Biotinylated Human BCMA, His Tag, Avi TagBiotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4) ELISA bioactivity

Immobilized Anti-Human BCMA MAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4) with a linear range of 0.5-8 ng/mL (Routinely tested).

Biotinylated Human BCMA, His Tag, Avi TagBiotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4) ELISA bioactivity

Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4) with a linear range of 0.6-20 ng/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-BCMA CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-BCMA-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4, 1 μg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-BCMA-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human BCMA, His Tag, Avi Tag (Cat. No. BCA-H82E4).

  • Background
    Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $1380.00

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