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Your Position: Home > Immune Checkpoint Proteins > CD155 > CD5-H82E3

Biotinylated Human CD155 / PVR Protein, His,Avitag™

  • Synonym
    PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
  • Source
    Biotinylated Human CD155, His,Avitag (CD5-H82E3) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.4).
    Predicted N-terminus: Trp 21
  • Molecular Characterization
    Online(Trp 21 - Asn 343) NP_006496.4

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 38.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) SDS-PAGE gel

Biotinylated Human CD155, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD155, His,AvitagBiotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) ELISA bioactivity

Immobilized Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) with a linear range of 0.078-1.25 μg/mL (QC tested).

Biotinylated Human CD155, His,AvitagBiotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) ELISA bioactivity

Immobilized Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) at 5 μg/mL (100 μL/well) via streptavidin can bind Human TIGIT, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.1-2 ng/mL (Routinely tested).

Biotinylated Human CD155, His,AvitagBiotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) ELISA bioactivity

Immobilized Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) at 5 μg/mL (100 μL/well) via streptavidin can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 0.1-1 ng/mL (Routinely tested).

  • Background
    CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:0 Details
  • Latest Research Phase:Discontinued

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DMF (Drug Master File)

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