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Your Position: Home > Immune Checkpoint Proteins > CD155 > Biotinylated Human CD155 / PVR Protein, His,Avitag

Biotinylated Human CD155 / PVR Protein, His,Avitag™

  • Synonym
    PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
  • Source
    Biotinylated Human CD155, His,Avitag (CD5-H82E3) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.4).
    Predicted N-terminus: Trp 21
  • Molecular Characterization
    Online(Trp 21 - Asn 343) NP_006496.4

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 38.8 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) SDS-PAGE gel

Biotinylated Human CD155, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD155, His,AvitagBiotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) ELISA bioactivity

Immobilized Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) with a linear range of 0.078-1.25 μg/mL (QC tested).

Biotinylated Human CD155, His,AvitagBiotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) ELISA bioactivity

Immobilized Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) at 5 μg/mL (100 μL/well) via streptavidin can bind Human TIGIT, Fc Tag (Cat. No. TIT-H5254) with a linear range of 0.1-2 ng/mL (Routinely tested).

Biotinylated Human CD155, His,AvitagBiotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) ELISA bioactivity

Immobilized Biotinylated Human CD155, His,Avitag (Cat. No. CD5-H82E3) at 5 μg/mL (100 μL/well) via streptavidin can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 0.1-1 ng/mL (Routinely tested).

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