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Your Position: Home > Receptor Tyrosine Kinases > Her2 > Biotinylated Human Her2 / ErbB2 Protein, His Tag, Avi Tag

Biotinylated Human Her2 / ErbB2 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    ERBB2,CD340,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1
  • Source
    Biotinylated Human Her2, His Tag, Avi Tag (HE2-H82E2) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).
    Predicted N-terminus: Thr 23
  • Molecular Characterization
    Online(Thr 23 - Thr 652) P04626-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 73.0 kDa. The protein migrates as 85-95 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human Her2, His Tag, Avi Tag (Cat. No. HE2-H82E2) SDS-PAGE gel

Biotinylated Human Her2, His Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human Her2, His Tag, Avi TagBiotinylated Human Her2, His Tag, Avi Tag (Cat. No. HE2-H82E2) ELISA bioactivity

Immobilized Trastuzumab at 3 μg/mL (100 μL/well) can bind Biotinylated Human Her2, His Tag, Avi Tag (Cat. No. HE2-H82E2) with a linear range of 0.2-6 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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Her2 Products

  • Name:Receptor protein-tyrosine kinase erbB-2
  • Classification:Homo sapiens
  • Number of Launched Drugs:20 Details
  • Number of Drugs in Clinical Trials:152 Details
  • Lastest Research Phase:Approved
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