This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.1 kDa respectively. The protein migrates as 17 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>97% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.
Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 4.39 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS analysis shows that Human PCSK9, His Tag (Cat. No. PC9-H5223) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 0.2857-0.4668 μg/mL.
Authors: M Wijers, et al
Journal: JCI Insight 2019
Application: Tissue culture
Authors: Lichtor PA, et al
Journal: Nat Chem Biol 2019
Authors: J Rohloff et al.
Journal: US9938314B2 2018
Authors: Grin PM, et al.
Journal: Sci Rep 2018
Application: Cell Culture
Authors: Chen Z, et al.
Journal: Nat Chem 2018
Application: PCSK9-binding Selection
Authors: Topchiy E, et al.
Journal: PLoS One 2016
Authors: Boyd JH, et al.
Journal: Journal of Innate Immunity 2016
Authors: Walley KR, et al.
Journal: Sci Transl Med 2014
Authors: Ledbetter, et al.
Journal: US20140120091A1 2014
Application: Capture ELISA
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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