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Human PCSK9 Protein, His Tag

  • Synonym
  • Source
    Human PCSK9, His Tag (PC9-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    Online(Gln 31 - Gln 692) Q8NBP7-1

    This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.1 kDa respectively. The protein migrates as 17 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >97% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 24 months under sterile conditions after reconstitution.
Human PCSK9, His Tag (Cat. No. PC9-H5223) SDS-PAGE gel

Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Human PCSK9, His TagHuman PCSK9, His Tag (Cat. No. PC9-H5223) ELISA bioactivity

Immobilized Human LDL R, Fc Tag (Cat. No. LDR-H5254) at 10 μg/mL (100 μL/well) can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with a linear range of 20-313 ng/mL (QC tested).

Human PCSK9, His TagHuman PCSK9, His Tag (Cat. No. PC9-H5223) ELISA bioactivity

Immobilized Human PCSK9, His Tag (Cat. No. PC9-H5223) at 1 μg/mL (100 μL/well) on His Tag Antibody, mAb, Mouse precoated (0.1 μg/well) plate, can bind PCSK9 Mab, Human IgG1 with a linear range of 0.2-3 ng/mL (Routinely tested).


Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 4.39 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that Human PCSK9, His Tag (Cat. No. PC9-H5223) inhibits LDL uptake in HepG2 cells. The EC50 for this effect is 0.2857-0.4668 μg/mL.

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