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Your Position: Home > Bio-Markers & CD Antigens > Human-PCSK9-Protein

Human PCSK9 Protein

  • Synonym
    PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
  • Source
    Human PCSK9, His Tag (PC9-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # AAI66619).
    Predicted N-terminus: Gln 31
  • Molecular Characterization
    PCSK9(Gln 31 - Gln 692) AAI66619

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 75.1 kDa. The protein migrates as 20 kDa and 62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >97% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human PCSK9, His Tag (Cat. No. PC9-H5223) SDS-PAGE gel

Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Bioactivity
Human PCSK9, His TagHuman PCSK9, His Tag (Cat. No. PC9-H5223) ELISA bioactivity

Immobilized Human LDL R, His Tag (Cat. No. LDR-H5224) at 10 μg/mL (100 μL/well) can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with a linear range of 0.031-0.125 μg/mL (QC tested).

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