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Your Position: Home > Receptor Tyrosine Kinases > VEGF R1 > Biotinylated Human VEGF R1 / Flt-1 Protein, His,Avitag

Biotinylated Human VEGF R1 / Flt-1 Protein, His,Avitag™

  • Synonym
    FLT,VEGFR1,FLT1
  • Source
    Biotinylated Human VEGF R1, His,Avitag (VE1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 27 - Asn 756 (Accession # P17948-1).
    Predicted N-terminus: Ser 27
  • Molecular Characterization
    Online(Ser 27 - Asn 756) P17948-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 85.9 kDa. The protein migrates as 106-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) SDS-PAGE gel

Biotinylated Human VEGF R1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human VEGF R1, His,AvitagBiotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) ELISA bioactivity

Immobilized Human VEGF165, Tag Free (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) with a linear range of 1-31 ng/mL (QC tested).

Biotinylated Human VEGF R1, His,AvitagBiotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) ELISA bioactivity

Immobilized Human PLGF, His Tag (Cat. No. PGF-H5229) at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) with a linear range of 2-20 ng/mL (Routinely tested).

Biotinylated Human VEGF R1, His,AvitagBiotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) ELISA bioactivity

Immobilized Human VEGF-C, His Tag (Cat. No. VEC-H4225) at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R1, His,Avitag (Cat. No. VE1-H82E3) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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