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Your Position: Home > All Other Proteins > ANGPTL7 > Biotinylated Human ANGPTL7 / CDT6 Protein, Mouse IgG2a Fc Tag, Avi Tag

Biotinylated Human ANGPTL7 / CDT6 Protein, Mouse IgG2a Fc Tag, Avi Tag (Avitag™)

  • Synonym
    ANGPTL7,CDT6
  • Source
    Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (AN7-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).
    Predicted N-terminus: Gln 27
  • Molecular Characterization
    ANGPTL7(Gln 27 - Pro 346) O43827-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 66.5 kDa. The protein migrates as 66 kDa and 75-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) SDS-PAGE gel

Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi TagBiotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) ELISA bioactivity

Immobilized Human LILRB4, Fc Tag (Cat. No. LI4-H5259) at 1 μg/mL (100 μL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) with a linear range of 0.078-2.5 μg/mL (QC tested).

Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi TagBiotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) ELISA bioactivity

Immobilized Human LILRB2, Fc Tag (Cat. No. CDD-H5259) at 1 μg/mL (100 μL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) with a linear range of 0.078-1.25 μg/mL (Routinely tested).

Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi TagBiotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) ELISA bioactivity

Immobilized Human LILRA3, Fc Tag (Cat. No. LI3-H5256) at 1 μg/mL (100 μL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) with a linear range of 0.078-5 μg/mL (Routinely tested).

Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi TagBiotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) ELISA bioactivity

Immobilized Human LILRB5, His Tag (Cat. No. CDC-H5220) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) with a linear range of 20-625 ng/mL (Routinely tested).

  • Background
    Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $1380.00

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