0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > ANGPTL7 > Biotinylated Human ANGPTL7 / CDT6 Protein, Mouse IgG2a Fc Tag, Avi Tag

Biotinylated Human ANGPTL7 / CDT6 Protein, Mouse IgG2a Fc Tag, Avi Tag (Avitag™)

  • Synonym
    ANGPTL7,CDT6
  • Source
    Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (AN7-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).
    Predicted N-terminus: Gln 27
  • Molecular Characterization
    Online(Gln 27 - Pro 346) O43827-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 65.8 kDa. The protein migrates as 66 kDa and 75-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) SDS-PAGE gel

Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi TagBiotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) ELISA bioactivity

Immobilized Human LILRB2, Fc Tag (Cat. No. CDD-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ANGPTL7, Mouse IgG2a Fc Tag, Avi Tag (Cat. No. AN7-H82F3) with a linear range of 0.156-2.5 μg/mL (QC tested).

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

Promotion & Exhibitions

  • PEGS Boston 2018
  • WPC Boston 2018

Datasheet & Documentation

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop