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Your Position: Home > Recombinant Proteins > LILRA2 > Biotinylated Human LILRA2 / CD85h / ILT1 Protein, His Tag, Avi Tag

Biotinylated Human LILRA2 / CD85h / ILT1 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    ILT1, LIR7, CD85H, LIR-7, LILRA2, ILT1CD85H, CD85h, ILT1, ILT-1, ILT1CD85H, CD85h antigen, CD85 antigen-like family member H
  • Source
    Biotinylated Human LILRA2, His Tag, Avi Tag (LI2-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Asn 449 (Accession # Q8N149-1).
    Predicted N-terminus: Gly 24
  • Molecular Characterization
    LILRA2(Gly 24 - Asn 449) Q8N149-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 50.7 kDa. The protein migrates as 67-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LILRA2, His Tag, Avi Tag (Cat. No. LI2-H82E9) SDS-PAGE gel

Biotinylated Human LILRA2, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Biotinylated Human LILRA2, His Tag, Avi TagBiotinylated Human LILRA2, His Tag, Avi Tag (Cat. No. LI2-H82E9) ELISA bioactivity

Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LILRA2, His Tag, Avi Tag (Cat. No. LI2-H82E9) with a linear range of 0.625-5 μg/mL (QC tested).

  • Background
    LILRA2 (Leukocyte immunoglobulin like receptor A2), also known as CD85H and LIR7 (Leukocyte immunoglobulin-like receptor 7). It belongs to the subfamily A class of LIR receptors (LILRAs), which are single-pass type I transmembrane proteins with 2-4 extracellular Ig-like domains, a transmembrane domain (TM), and a short cytoplasmic tail. LILRA2 contains 4 Ig-like C2 type domains in the extracellular region. LILRA2 does not bind class I MHC antigens. LILRA2 is expressed predominantly on monocytes and B cells, and at lower levels on dendritic cells and natural killer cells. LILRA2 is an activating receptor that inhibits dendritic cell differentiation and antigen presentation and suppresses innate immune response.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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