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Your Position: Home > All Other Proteins > CD19 > CD9-H5250

Human CD19 (20-291) Protein, Llama IgG2b Fc Tag, low endotoxin

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    CD19,B4,CVID3,MGC12802
  • Source
    Human CD19 (20-291), Llama IgG2b Fc Tag, low endotoxin (CD9-H5250) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).
    Predicted N-terminus: Pro 20
    Request for sequence
  • Molecular Characterization
    Online(Pro 20 - Lys 291) P15391-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 57.9 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD19 (20-291), Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD9-H5250) SDS-PAGE gel

Human CD19 (20-291), Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD19 (20-291), Llama IgG2b Fc Tag, low endotoxinHuman CD19 (20-291), Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD9-H5250) ELISA bioactivity

Immobilized FMC63 at 0.5 μg/mL (100 μL/well) can bind Human CD19 (20-291), Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD9-H5250) with a linear range of 0.078-1.25 μg/mL (QC tested).

Request for protocol
Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Human CD19 (20-291), Llama IgG2b Fc Tag, low endotoxin (Cat. No. CD9-H5250) on CM5 chip can bind FMC63 MAb (mouse lgG2a) with an affinity constant of 0.628 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Request for protocol
  • Background
    B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I  membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.
  • References

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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