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Your Position: Home > All Other Proteins > LILRB4 > Human LILRB4 / CD85k / ILT3 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human LILRB4 / CD85k / ILT3 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    LILRB4,ILT3,LIR5,CD85K,HM18
  • Source
    Human LILRB4, Mouse IgG2a Fc Tag, low endotoxin (CDK-H5259) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Glu 259 (Accession # AAH26309.1).
    Predicted N-terminus: Gln 22
  • Molecular Characterization
    LILRB4(Gln 22 - Glu 259) AAH26309.1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 53.2 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human LILRB4, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDK-H5259) SDS-PAGE gel

Human LILRB4, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human LILRB4, Mouse IgG2a Fc Tag, low endotoxinHuman LILRB4, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDK-H5259) ELISA bioactivity

Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Human LILRB4, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDK-H5259) with a linear range of 0.313-2.5 μg/mL (QC tested).

  • Background
    Leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4) is also known as CD85 antigen-like family member K (CD85K), Immunoglobulin-like transcript 3 (ILT-3), Leukocyte immunoglobulin-like receptor 5 (LIR-5), Monocyte inhibitory receptor HM18, which belongs to the leukocyte immunoglobulin-like receptor (LIR) family. LILRB4 / CD85K contains 2 Ig-like C2-type (immunoglobulin-like) domains. CD85K is detected in monocytes, macrophages, dendritic cells, lung, natural killer cells and B-cells. LILRB4 / CD85K is receptor for class I MHC antigens. CD85K recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles, involved in the down-regulation of the immune response and the development of tolerance. LILRB4 interferes with TNFRSF5-signaling and NF-kappa-B up-regulation and inhibits receptor-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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