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Your Position: Home > All Other Proteins > TSLP > Human TSLP Protein

Human TSLP Protein

  • Synonym
    TSLP
  • Source
    Human TSLP (TSP-H52Hb) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).
    Predicted N-terminus: Tyr 29
  • Molecular Characterization
    TSLP(Tyr 29 - Gln 159) Q969D9-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.8 kDa. The protein migrates as 8 kDa and 14-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human TSLP (Cat. No. TSP-H52Hb) SDS-PAGE gel

Human TSLP on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human TSLPHuman TSLP (Cat. No. TSP-H52Hb) ELISA bioactivity

Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Human TSLP (Cat. No. TSP-H52Hb) with a linear range of 1.6-13 ng/mL (QC tested).

Human TSLPHuman TSLP (Cat. No. TSP-H52Hb) ELISA bioactivity

Immobilized Human TSLP (Cat. No. TSP-H52Hb) at 1 μg/mL (100 μL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.5-8 ng/mL (Routinely tested).

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