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Your Position: Home > Immune Checkpoint Proteins > CD27 Ligand > Mouse CD27 Ligand / CD70 Protein (active trimer) (HPLC-verified)

Mouse CD27 Ligand / CD70 Protein (active trimer) (HPLC-verified)

  • Synonym
    CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
  • Source
    Mouse CD27 Ligand (active trimer) (HPLC-verified) (CDL-M5245) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).
    Predicted N-terminus: His
  • Molecular Characterization
    CD27 Ligand(Gln 47 - Pro 195) O55237-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 18.6 kDa. The protein migrates as 27-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse CD27 Ligand (active trimer) (HPLC-verified) (Cat. No. CDL-M5245) SDS-PAGE gel

Mouse CD27 Ligand (active trimer) (HPLC-verified) on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Mouse CD27 Ligand (active trimer) (HPLC-verified) (Cat. No. ) HPLC images

A SEC-HPLC analysis showing over 95% of Mouse CD27 Ligand, His Tag (Cat. No. CDL-M5245)  present as active trimers with a molecular mass around 90 kDa.

Bioactivity-ELISA
Mouse CD27 Ligand (active trimer) (HPLC-verified)Mouse CD27 Ligand (active trimer) (HPLC-verified) (Cat. No. CDL-M5245) ELISA bioactivity

Immobilized Mouse CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Mouse CD27 Ligand (active trimer) (HPLC-verified) (Cat. No. CDL-M5245) with a linear range of 0.1-4 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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