>Human TNF-alpha Protein,HEK293 expressed,ActiveMax, low endotoxin
This protein carries no "tag".
The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa and 18 kDa under reducing (R) condition (SDS-PAGE).
A SEC-HPLC analysis showing 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active trimers with a molecular mass of 54 kDa.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Human TNF-alpha, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
A SEC-HPLC analysis showing over 95% of ActiveMax® Recombinant Human TNF-alpha (TNA-H4211) present as active trimers with a molecular mass of 54 kDa.
Immobilized Human TNF-alpha, Tag Free (Cat. No. TNA-H4211) at 2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.2-2 ng/mL (QC tested).
Immobilized Humira on CM5 Chip via anti-human Fc IgG, can bind Human TNF-alpha Protein (Cat.No. TNA-H4211) with an affinity constant of 0.114nM as determined in SPR assay (Biacore T200) ( Routinely tested).
Recombinant Human TNF-alpha (Cat.No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014ng/ml (Routinely tested).
Neutralization assay shows that the cytotoxicity effect of TNF-alpha (Cat.No. TNA-H4211) was inhibited by increasing concentration of Adalimumab. The concentration of TNF-alpha used is 1ng/mL. The IC50 is 29ng/mL (Routinely tested).
Authors: A.E. Ross, et al.
Journal: ANAL CHIM ACTA 2017
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