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Your Position: Home > All Other Proteins > RANK > Human RANK / TNFRSF11A Protein, Mouse IgG2a Fc Tag, low endotoxin

Human RANK / TNFRSF11A Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD265, FEO, LOH18CR1, ODFR, OFE, OPTB7, OSTS, PDB2, RANK, TNFRSF11A, TRANCER, CD265 antigen, ODFROSTS,RANKLOH18CR1, NFKB activator
  • Source
    Human RANK, Mouse IgG2a Fc Tag, low endotoxin (RAK-H5251) is expressed from human 293 cells (HEK293). It contains AA Ile 30 - Pro 212 (Accession # Q9Y6Q6-1).
    Predicted N-terminus: Ile 30
  • Molecular Characterization
    RANK(Ile 30 - Pro 212) Q9Y6Q6-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 47.2 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) SDS-PAGE gel

Human RANK, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human RANK, Mouse IgG2a Fc Tag, low endotoxinHuman RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) ELISA bioactivity

Immobilized Human TNFSF11, His Tag (Cat. No. RAL-H5240) at 2 μg/mL (100 μL/well) can bind Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) with a linear range of 0.4-13 ng/mL (QC tested).

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Price(USD) : $2100.00

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