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Your Position: Home > Protein > TSLP > TSP-H52Ha

Human TSLP (R127A, R130A) Protein, His Tag (MALS verified)

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  • Synonym
    TSLP
  • Source
    Human TSLP (R127A, R130A) Protein, His Tag(TSP-H52Ha) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).
    Predicted N-terminus: Tyr 29
  • Molecular Characterization
    TSLP Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.7 kDa. The protein migrates as 25-29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
TSLP SDS-PAGE

Human TSLP (R127A, R130A) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
TSLP MALS images

The purity of Human TSLP (R127A, R130A) Protein, His Tag (Cat. No. TSP-H52Ha) is more than 90% and the molecular weight of this protein is around 18-26 kDa verified by SEC-MALS.

Bioactivity-ELISA
 TSLP ELISA

Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with a linear range of 3-50 ng/mL (QC tested).

 TSLP ELISA

Immobilized Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) at 1 μg/mL (100 μL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.4-3 ng/mL (Routinely tested).

Bioactivity-SPR
 TSLP SPR

Captured Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag (Cat. No. TSR-H525a) with an affinity constant of 4.22 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 TSLP BLI

Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with an affinity constant of 16 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 TSLP BLI

Loaded Monoclonal Anti-Human TSLP Antibody, Human IgG2 on AHC Biosensor, can bind Human TSLP (R127A, R130A), His Tag (Cat. No. TSP-H52Ha) with an affinity constant of 0.153 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
  • Clinical and Translational Updates

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