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Your Position: Home > Recombinant Proteins > TSLP > Human TSLP (R127A, R130A) Protein

Human TSLP (R127A, R130A) Protein

  • Synonym
    TSLP
  • Source
    Human TSLP (R127A, R130A) (TSP-H52Ha) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).
    Predicted N-terminus: Tyr 29
  • Molecular Characterization
    TSLP(Tyr 29 - Gln 159) Q969D9-1 (R127A, R130A)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.7 kDa. The protein migrates as 22-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human TSLP (R127A, R130A) (Cat. No. TSP-H52Ha) SDS-PAGE gel

Human TSLP (R127A, R130A) on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human TSLP (R127A, R130A)Human TSLP (R127A, R130A) (Cat. No. TSP-H52Ha) ELISA bioactivity

Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Human TSLP (R127A, R130A) (Cat. No. TSP-H52Ha) with a linear range of 0.8-6 ng/mL (QC tested).

Human TSLP (R127A, R130A)Human TSLP (R127A, R130A) (Cat. No. TSP-H52Ha) ELISA bioactivity

Immobilized Human TSLP (R127A, R130A) (Cat. No. TSP-H52Ha) at 1 μg/mL (100 μL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.4-3 ng/mL (Routinely tested).

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