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Your Position: Home > All Other Proteins > TNFSF11 > Human TNFSF11 / RANKL / CD254 Protein, Fc tag

Human TNFSF11 / RANKL / CD254 Protein, Fc tag

  • Synonym
    RANKL,CD254,TRANCE,OPGL,ODF
  • Source
    Human TNFSF11, Fc tag (RAL-H5265) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).
    Predicted N-terminus: Pro
  • Molecular Characterization
    TNFSF11(Gly 64 - Asp 245) AAC51762.1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 46.9 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human TNFSF11, Fc tag (Cat. No. RAL-H5265) SDS-PAGE gel

Human TNFSF11, Fc tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human TNFSF11, Fc tagHuman TNFSF11, Fc tag (Cat. No. RAL-H5265) ELISA bioactivity

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 0.5 μg/mL (100 μL/well) can bind Human TNFSF11, Fc tag (Cat. No. RAL-H5265) with a linear range of 0.1-3 ng/mL (QC tested).

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