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Your Position: Home > Immune Checkpoint Proteins > GITR > Biotinylated Human GITR / TNFRSF18 Protein, His Tag, Avi Tag

Biotinylated Human GITR / TNFRSF18 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    AITR,GITR,TNFRSF18,CD357
  • Source
    Biotinylated Human GITR, His Tag, Avi Tag (GIR-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    GITR(Gln 26 - Glu 161) Q9Y5U5-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 18.1 kDa. The protein migrates as 22-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human GITR, His Tag, Avi Tag (Cat. No. GIR-H82E4) SDS-PAGE gel

Biotinylated Human GITR, His Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human GITR, His Tag, Avi TagBiotinylated Human GITR, His Tag, Avi Tag (Cat. No. GIR-H82E4) ELISA bioactivity

Immobilized Human GITR Ligand, Fc Tag (Cat. No. GIL-H5261) at 10 μg/mL (100 μL/well) can bind Biotinylated Human GITR, His Tag, Avi Tag (Cat. No. GIR-H82E4) with a linear range of 1-78 ng/mL (QC tested).

  • Background
    Glucocorticoid-induced TNFR-related protein (GITR) is also known as Tumor necrosis factor receptor superfamily member 18 (TNFRSF18), activation-inducible TNFR family receptor (AITR), CD antigen CD357, which is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is receptor for TNFSF18, which seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. GITR also mediated NF-kappa-B activation via the TRAF2/NIK pathway.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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GITR Products

  • Name:Glucocorticoid-induced tumor necrosis factor receptor-related protein
  • Classification:Homo sapiens
  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:9 Details
  • Lastest Research Phase:Phase ?
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