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Your Position: Home > Immune Checkpoint Proteins > CD47 > Human CD47 Protein, low endotoxin

Human CD47 Protein, low endotoxin

  • Synonym
    CD47,MER6,IAP,OA3
  • Source
    Human CD47, His Tag, low endotoxin (CD7-H522a) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 139 (Accession # NP_942088).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    CD47(Gln 19 - Pro 139) NP_942088

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 15.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CD47, His Tag, low endotoxin (Cat. No. CD7-H522a) SDS-PAGE gel

Human CD47, His Tag, low endotoxin on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human CD47, His Tag, low endotoxinHuman CD47, His Tag, low endotoxin (Cat. No. CD7-H522a) ELISA bioactivity

Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag, low endotoxin (Cat. No. CD7-H522a) with a linear range of 2-13 ng/mL (QC tested).

Human CD47, His Tag, low endotoxinHuman CD47, His Tag, low endotoxin (Cat. No. CD7-H522a) ELISA bioactivity

Immobilized Anti-CD47 MAb, Human IgG4 (clone 5F9) at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag, low endotoxin (Cat. No. CD7-H522a) with a linear range of 0.4-3 ng/mL (Routinely tested).

  • Background
    Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $3000.00

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