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Your Position: Home > Cytokines > Prolactin > Biotinylated Human Prolactin / PRL Protein, Fc Tag, Avi Tag

Biotinylated Human Prolactin / PRL Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    PRL, Prolactin
  • Source
    Biotinylated Human Prolactin, Fc Tag, Avi Tag (PRN-H82F7) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Cys 227 (Accession # Q5THQ0-1).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    Prolactin(Leu 29 - Cys 227) Q5THQ0-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 51.2 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arg, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Prolactin, Fc Tag, Avi Tag  (Cat. No. PRN-H82F7) SDS-PAGE gel

Biotinylated Human Prolactin, Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Biotinylated Human Prolactin, Fc Tag, Avi Tag Biotinylated Human Prolactin, Fc Tag, Avi Tag  (Cat. No. PRN-H82F7) ELISA bioactivity

Immobilized Human Prolactin R / PRLR Protein, Fc Tag (Cat. No. PRP-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Prolactin, Fc Tag, Avi Tag (Cat. No. PRN-H82F7) with a linear range of 5-156 ng/mL (QC tested).

  • Background
    Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females. Prolactin is synthesized as a prohormone. Following cleavage of the signal peptide, the length of the mature hormone is between 194 and 199 amino acids, depending on species. Hormone structure is stabilized by three intramolecular disulfide bonds. Excessive secretion of prolactin - hyperprolactinemia - is a relative common disorder in humans. This condition has numerous causes, including prolactin-secreting tumors and therapy with certain drugs. The prolactin receptor (gene name PRLR) is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. Expression of the prolactin receptor is widespread. Each prolactin molecule is thought to bind two receptor molecules
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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