0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > SIRP alpha > Cynomolgus SIRP alpha / CD172a Protein

Cynomolgus SIRP alpha / CD172a Protein

  • Synonym
    SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
  • Source
    Cynomolgus SIRP alpha (SIA-C52H7) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 370 (Accession # NP_001271679.1).
    Predicted N-terminus: Glu 31
  • Molecular Characterization
    SIRP alpha(Glu 31 - Asn 370) NP_001271679.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.0 kDa. The protein migrates as 66 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Cynomolgus SIRP alpha (Cat. No. SIA-C52H7) SDS-PAGE gel

Cynomolgus SIRP alpha on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Cynomolgus SIRP alphaCynomolgus SIRP alpha (Cat. No. SIA-C52H7) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque CD47, Fc Tag (Cat. No. CD7-C5252) at 2 μg/mL (100 μL/well) can bind Cynomolgus SIRP alpha (Cat. No. SIA-C52H7) with a linear range of 0.313-2.5 μg/mL (QC tested).

  • Background
    Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

    Comments (0)

    Order Details

    Price(USD) : $300.00

    Price(USD) : $1980.00

    Promotion & Exhibitions

    • PEGS Boston 2018
    • WPC Boston 2018

    Datasheet & Documentation

    Contact Us

    +1  800-810-0816 (US)
    +86 400-682-2521 (AP)

    Questions & Comments

    This web search service is supported by Google Inc.

    totop