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Your Position: Home > Recombinant Proteins > Integrin alpha 4 beta 1 > Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein

Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha 4 beta 1,ITGA4&ITGB1
  • Source
    Recombinant Human ITGA4 & ITGB1 Heterodimer Protein (IT1-H52W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
    Predicted N-terminus: Tyr 34 (ITGA4) & Gln 21 (ITGB1)
  • Molecular Characterization
    ITGA4&ITGB1(Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)) P13612-1 (ITGA4) & P05556-1 (ITGB1)

    Human ITGA4 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB1). Subunit ITGA4 is  fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Tyr 34 (ITGA4) & Gln 21 (ITGB1). The reducing (R) protein migrates as 60-70 kDa and 80-85 kDa (ITGA4) and 100-133 kDa and 135-150 kDa (ITGB1) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human ITGA4 & ITGB1 (Cat. No. IT1-H52W1) SDS-PAGE gel

Human ITGA4 & ITGB1 on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human ITGA4 & ITGB1Human ITGA4 & ITGB1 (Cat. No. IT1-H52W1) ELISA bioactivity

Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Human ITGA4 & ITGB1 (Cat. No. IT1-H52W1) with a linear range of 1-16 ng/mL (QC tested).

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