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Your Position: Home > All Other Proteins > PLGF > Mouse PLGF / PGF Protein

Mouse PLGF / PGF Protein

  • Synonym
    PGF,PLGF,PlGF2,PlGF,PGFL,SHGC-10760
  • Source
    Mouse PLGF (PGF-M52H0) is expressed from human 293 cells (HEK293). It contains AA Val 19 - Pro 158 (Accession # P49764-1).
    Predicted N-terminus: Val 19
  • Molecular Characterization
    PLGF(Val 19 - Pro 158) P49764-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 17.8 kDa. The protein migrates as 27-35 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 100 mM HAC, pH2.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse PLGF (Cat. No. PGF-M52H0) SDS-PAGE gel

Mouse PLGF on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse PLGFMouse PLGF (Cat. No. PGF-M52H0) ELISA bioactivity

Immobilized Mouse VEGF R1, mouse IgG2a Fc Tag (Cat. No. VE1-M5256) at 2 μg/mL (100 μL/well) can bind Mouse PLGF (Cat. No. PGF-M52H0) with a linear range of 0.2-4 ng/mL (QC tested).

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