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Your Position: Home > All Other Proteins > CA125 > Biotinylated Human CA125 / MUC16 Protein, Fc Tag, Avi Tag

Biotinylated Human CA125 / MUC16 Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    CA125, CA-125, CA125MUC-16, FLJ14303, MUC16, mucin 16, mucin-16
  • Source
    Biotinylated Human CA125, Fc Tag, Avi Tag (CA5-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gly 12660 - Met 12923 (Accession # Q8WXI7-1).
    Predicted N-terminus: Gly 12660
  • Molecular Characterization
    CA125(Gly 12660 - Met 12923) Q8WXI7-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CA125, Fc Tag, Avi Tag (Cat. No. CA5-H82F4) SDS-PAGE gel

Biotinylated Human CA125, Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CA125, Fc Tag, Avi TagBiotinylated Human CA125, Fc Tag, Avi Tag (Cat. No. CA5-H82F4) ELISA bioactivity

Immobilized Human Mesothelin, His Tag (Cat. No. MSN-H5223) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc Tag, Avi Tag (Cat. No. CA5-H82F4) with a linear range of 2-78 ng/mL (QC tested).

  • Background
    The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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