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Your Position: Home > All Other Proteins > CA125 > Biotinylated Human CA125 / MUC16 Protein, Fc Tag, Avi Tag

Biotinylated Human CA125 / MUC16 Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    CA125, CA-125, CA125MUC-16, FLJ14303, MUC16, mucin 16, mucin-16
  • Source
    Biotinylated Human CA125, Fc Tag, Avi Tag (CA5-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gly 12660 - Met 12923 (Accession # Q8WXI7-1).
    Predicted N-terminus: Gly 12660
  • Molecular Characterization
    CA125(Gly 12660 - Met 12923) Q8WXI7-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CA125, Fc Tag, Avi Tag (Cat. No. CA5-H82F4) SDS-PAGE gel

Biotinylated Human CA125, Fc Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CA125, Fc Tag, Avi TagBiotinylated Human CA125, Fc Tag, Avi Tag (Cat. No. CA5-H82F4) ELISA bioactivity

Immobilized Human Mesothelin, His Tag (Cat. No. MSN-H5223) at 10 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc Tag, Avi Tag (Cat. No. CA5-H82F4) with a linear range of 2-78 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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