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Your Position: Home > All Other Proteins > LIGHT > Human LIGHT / TNFSF14 Protein, Fc tag

Human LIGHT / TNFSF14 Protein, Fc tag

  • Synonym
    CD258 antigen, CD258, HVEM-L, LIGHT, LTg, TNFSF14, TR2, HVEML
  • Source
    Human LIGHT, Fc tag (LIT-H5265) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1).
    Predicted N-terminus: Pro
  • Molecular Characterization
    LIGHT(Asp 74 - Val 240) O43557-1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 44.7 kDa. The protein migrates as 50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human LIGHT, Fc tag (Cat. No. LIT-H5265) SDS-PAGE gel

Human LIGHT, Fc tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
Human LIGHT, Fc tagHuman LIGHT, Fc tag (Cat. No. LIT-H5265) ELISA bioactivity

Immobilized Human HVEM, His Tag (Cat. No. HVM-H52E9) at 5 μg/mL (100 μL/well) can bind Human LIGHT, Fc tag (Cat. No. LIT-H5265) with a linear range of 0.5-8 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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