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Your Position: Home > Immune Checkpoint Proteins > LIGHT > Human LIGHT / TNFSF14 Protein, His Tag

Human LIGHT / TNFSF14 Protein, His Tag

  • Synonym
    CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
  • Source
    Human LIGHT, His Tag (LIT-H5247) is expressed from human 293 cells (HEK293). It contains AA Asp 74 - Val 240 (Accession # O43557-1).
  • Molecular Characterization
    Online(Asp 74 - Val 240) O43557-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 20.8 kDa. The protein migrates as 23-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LIGHT, His Tag (Cat. No. LIT-H5247) SDS-PAGE gel

Human LIGHT, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human LIGHT, His TagHuman LIGHT, His Tag (Cat. No. LIT-H5247) ELISA bioactivity

Immobilized Human HVEM, Fc Tag (Cat. No. HVM-H5258) at 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (Cat. No. LIT-H5247) with a linear range of 1-10 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human DcR3, Fc Tag (Cat. No. TNB-H5255) on AHC Biosensor, can bind Human LIGHT, His Tag (Cat. No. LIT-H5247) with an affinity constant of 4.94 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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