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Your Position: Home > Immune Checkpoint Proteins > CD28 > Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc Tag (HPLC-verified)

Human / Cynomolgus / Rhesus macaque CD28 Protein, Fc Tag (HPLC-verified)

  • Synonym
    CD28,Tp44
  • Source
    Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (CD8-H525a) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1).
    Predicted N-terminus: Asn 19
  • Molecular Characterization
    Online(Asn 19 - Pro 152) P10747-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) SDS-PAGE gel

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC
Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. ) HPLC images

The purity of Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA
Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified)Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-2, Fc Tag (Cat. No. CD6-H82F5) with a linear range of 0.019-0.625 μg/mL (QC tested).

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified)Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) at 2 μg/mL (100 μL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/ml (QC tested).

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