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>Integrin alpha V beta 3
>Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, His,Avitag&Tag Free
Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 114.7 kDa (ITGAV) and 81.8 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 (ITGAV) and 95-100 kDa (ITGB3) respectively due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82Wa) with a linear range of 0.078-1.25 μg/mL (QC tested).
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Price(USD) : $350.00
Price(USD) : $1380.00
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