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Your Position: Home > All Other Proteins > Integrin alpha V beta 3 > Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein

Biotinylated Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 3, ITGAV&ITGB3
  • Source
    Biotinylated Human ITGAV & ITGB3 Heterodimer Protein (IT3-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3) (Accession # NP_002201.1 (ITGAV) & P05106-1 (ITGB3)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gly 27 (ITGB3)
  • Molecular Characterization
    ITGAV&ITGB3(Phe 31 - Val 992 (ITGAV) & Gly 27 - Asp 718 (ITGB3)) NP_002201.1 (ITGAV) & P05106-1 (ITGB3)

    Human ITGAV & ITGB3 Heterodimer Protein, produced by co-expression of ITGAV and ITGB3, has a calculated MW of 114.7 kDa (ITGAV) and 81.8 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB3 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Gly 27 (ITGB3). The reducing (R) protein migrates as 120-130 kDa and 145-150 (ITGAV) and 95-100 kDa (ITGB3) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 2 mM CaCl2, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H82Wa) SDS-PAGE gel

Biotinylated Human ITGAV & ITGB3 Heterodimer Protein on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Biotinylated Human ITGAV & ITGB3 Heterodimer ProteinBiotinylated Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H82Wa) ELISA bioactivity

Immobilized Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H82Wa) with a linear range of 0.078-1.25 μg/mL (QC tested).

Biotinylated Human ITGAV & ITGB3 Heterodimer ProteinBiotinylated Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H82Wa) ELISA bioactivity

Immobilized Vitronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB3 Heterodimer Protein (Cat. No. IT3-H82Wa) with a linear range of 0.078-1.25 μg/mL (Routinely tested).

  • Background
    Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Also, Integrin alpha-V/beta-3 acts as a receptor for herpes virus 8/HHV-8, coxsackievirus A9, Hantaan virus, cytomegalovirus/HHV-5, human metapneumovirus, human parechovirus 1 and west nile virus. Furthermore, in case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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