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Your Position: Home > All Other Proteins > Integrin alpha 4 beta 7 > Biotinylated Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein

Biotinylated Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein

  • Synonym
    Integrin alpha 4 beta 7,ITGA4&ITGB7
  • Source
    Biotinylated Human ITGA4 & ITGB7 Heterodimer Protein (IT7-H82W9) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).
    Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
  • Molecular Characterization
    Online(Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7)) P13612-1 (ITGA4) & P26010-1 (ITGB7)

    Human ITGA4 & ITGB7 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 113.2 kDa (ITGA4) & 81.8 kDa (ITGB7). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 110 kDa and 130 kDa (ITGA4) and 60-70 kDa (ITGB7) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ITGA4 & ITGB7 Heterodimer Protein (Cat. No. IT7-H82W9) SDS-PAGE gel

Biotinylated Human ITGA4 & ITGB7 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human ITGA4 & ITGB7 Heterodimer ProteinBiotinylated Human ITGA4 & ITGB7 Heterodimer Protein (Cat. No. IT7-H82W9) ELISA bioactivity

Immobilized Anti-alpha4 unit at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4 & ITGB7 Heterodimer Protein (Cat. No. IT7-H82W9) with a linear range of 4-63 ng/mL (QC tested).

  • Background
    Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 . Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes.Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid. The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT). Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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