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Your Position: Home > Protein > M-CSF > MCF-H82E6

Biotinylated Human M-CSF / CSF-1 Protein, His,Avitag™

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  • Synonym
    M-CSF,CSF-1,Lanimostim
  • Source
    Biotinylated Human M-CSF, His,Avitag(MCF-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1 ).
    Predicted N-terminus: Glu 33
  • Molecular Characterization
    M-CSF Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 28.8 kDa. The protein migrates as 35-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
M-CSF SDS-PAGE

Biotinylated Human M-CSF, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 M-CSF ELISA

Immobilized Human M-CSF R, Fc Tag (Cat. No. CSR-H5258) at 2 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) with a linear range of 0.1-2 ng/mL (QC tested).

 M-CSF ELISA

Immobilized Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Cynomolgus M-CSF R, Fc Tag (Cat. No. CSR-C5252) with a linear range of 1-39 ng/mL (Routinely tested).

Bioactivity-SPR
 M-CSF SPR

Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. CSR-H5228) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI
 M-CSF BLI

Loaded Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) on SA Biosensor, can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. CSR-H5258) with an affinity constant of 24.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Bioactivity CELL BASE
 M-CSF CELL

Biotinylated Human M-CSF, His,Avitag (Cat. No. MCF-H82E6) stimulates proliferation of RAW264.7 cells. The EC50 for this effect is 6.20-7.15 ng/mL (Routinely tested).

 M-CSF CELL

The bio-activity of Biotinylated Human M-CSF / CSF-1 Protein, His,Avitag™ (Cat. No. MCF-H82E6) was determined by dose-dependent stimulation of the proliferation of M-NFS-60 cells. The EC50 for this effect is 10.91ng/mL (Routinely tested).

  • Background
    The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.
  • Clinical and Translational Updates

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