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Your Position: Home > Recombinant Proteins > Integrin alpha 6 beta 1 > Biotinylated Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein

Biotinylated Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein

This product is still underdevelopment. Please contact us if you have interest in this product. We will accelerate the development process accordingly and reserve this product for you as request.
  • Synonym
    Integrin alpha 6 beta 1, ITGA6&ITGB1
  • Source
    Biotinylated Human ITGA6 & ITGB1 Heterodimer Protein (IT1-H82W8) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1) (Accession # P23229-2 (ITGA6) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 24 (ITGA6) & Gln 21 (ITGB1)
  • Molecular Characterization
    ITGA6B1(Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1)) P23229-2 (ITGA6) & P05556-1 (ITGB1)

    Human ITGA6 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA6 and ITGB1, has a calculated MW of 117.0 kDa (ITGA6) and 84.0 kDa (ITGB1). Subunit ITGA6 is  fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

  • Background
    Integrin alpha 6 beta 1, also called platelet glycoprotein GPIc-IIa, is a receptor for laminin on platelets. The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. ITGA6 has been shown to interact with TSPAN4 and GIPC1.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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