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Your Position: Home > Recombinant Proteins > Integrin alpha V beta 5 > Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein

Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein

  • Synonym
    Integrin alpha V beta 5, ITGAV&ITGB5
  • Source
    Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (IT5-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # NP_002201.1 (ITGAV) & P18084-1 (ITGB5)).
    Predicted N-terminus: Phe 31 (ITGAV) & Gly 24 (ITGB5)
  • Molecular Characterization
    ITGAV&ITGB5(Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5)) NP_002201.1 (ITGAV) & P18084-1 (ITGB5)

    Biotinylated Human ITGAV & ITGB5 Heterodimer Protein, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 120 kDa and 150kDa (ITGAV) and 90 kDa (ITGB5) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) SDS-PAGE gel

Biotinylated Human ITGAV & ITGB5 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human ITGAV & ITGB5 Heterodimer ProteinBiotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) ELISA bioactivity

Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) with a linear range of 0.01-0.156 μg/mL (QC tested).

Biotinylated Human ITGAV & ITGB5 Heterodimer ProteinBiotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) ELISA bioactivity

Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) with a linear range of 0.156-5 μg/mL (Routinely tested).

  • Background
    Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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