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>Integrin alpha V beta 5
>Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein
Biotinylated Human ITGAV & ITGB5 Heterodimer Protein, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB5 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 120 kDa and 150kDa (ITGAV) and 90 kDa (ITGB5) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human ITGAV & ITGB5 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) with a linear range of 0.01-0.156 μg/mL (QC tested).
Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein (Cat. No. IT5-H82Wa) with a linear range of 0.156-5 μg/mL (Routinely tested).
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.
Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $1380.00
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