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Your Position: Home > All Other Proteins > Integrin alpha 4 beta 1 > IT1-H82W1

Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His,Avitag™&Tag Free

  • Synonym
    Integrin alpha 4 beta 1,ITGA4&ITGB1
  • Source
    Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (IT1-H82W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
    Predicted N-terminus: Tyr 34 & Ser 592 (ITGA4) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)) P13612-1 (ITGA4) & P05556-1 (ITGB1)

    Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 52.1 kDa). Consequently ITGA4 migrates as 65-75 kDa, 80-85 kDa and 135-150 kDa, and ITGB1 as 100-133 kDa respectively under reducing (R) condition due to cleavage and glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) SDS-PAGE gel

Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) ELISA bioactivity

Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 2-31 ng/mL (QC tested).

Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) ELISA bioactivity

Immobilized Human VCAM-1, His Tag (Cat. No. VC1-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 0.01-0.156 μg/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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