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Your Position: Home > All Other Proteins > Integrin alpha 4 beta 1 > Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein

Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha 4 beta 1,ITGA4&ITGB1
  • Source
    Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (IT1-H82W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
    Predicted N-terminus: Tyr 34 (ITGA4) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)) P13612-1 (ITGA4) & P05556-1 (ITGB1)

    Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) & 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus.The reducing (R) protein migrates as 100-150 kDa (ITGA4) and 65-85 kDa (ITGB1) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) SDS-PAGE gel

Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human ITGA4 & ITGB1 Heterodimer ProteinBiotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) ELISA bioactivity

Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) with a linear range of 2-31 ng/mL (QC tested).

Biotinylated Human ITGA4 & ITGB1 Heterodimer ProteinBiotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) ELISA bioactivity

Immobilized Human VCAM-1, His Tag (Cat. No. VC1-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) with a linear range of 0.01-0.156 μg/mL (Routinely tested).

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