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Your Position: Home > All Other Proteins > Integrin alpha 4 beta 1 > Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein

Biotinylated Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha 4 beta 1, ITGA4&ITGB1
  • Source
    Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (IT1-H82W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
    Predicted N-terminus: Tyr 34 (ITGA4) & Gln 21 (ITGB1)
  • Molecular Characterization
    ITGA4&ITGB1(Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)) P13612-1 (ITGA4) & P05556-1 (ITGB1)

    Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 113.2 kDa (ITGA4) & 83.7 kDa (ITGB1). Subunit ITGA4 is  fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus.The reducing (R) protein migrates as 100-150 kDa (ITGA4) and 65-85 kDa (ITGB1) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) SDS-PAGE gel

Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human ITGA4 & ITGB1 Heterodimer ProteinBiotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) ELISA bioactivity

Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82W1) with a linear range of 2-31 ng/mL (QC tested).

  • Background
    Integrins are transmembrane proteins that mediate interactions between adhesion molecules on adjacent cells and/or the extracellular matrix (ECM). Integrins have diverse roles in several biological processes including cell migration during development and wound healing, cell differentiation, and apoptosis. Their activities can also regulate the metastatic and invasive potential of tumor cells. Integrin alpha 4 beta 1(Alpha-4/beta-1) is receptors for fibronectin. Integrin alpha-4/beta-1 is a receptor for VCAM1 and recognizes the sequence Q-I-D-S in VCAM1.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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