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Your Position: Home > Cytokines > CTGF > Human CTGF / CCN2 Protein, His Tag

Human CTGF / CCN2 Protein, His Tag

  • Synonym
    CCN2,NOV2,HCS24,IGFBP8,IBP-8,IGFBP-8,IGF-binding protein 8
  • Source
    Human CTGF, His Tag (CTF-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1).
    Predicted N-terminus: Gln 27
  • Molecular Characterization
    Online(Gln 27 - Ala 349) Q5M8T4-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 37.4 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, pH7.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CTGF, His Tag (Cat. No. CTF-H52H5) SDS-PAGE gel

Human CTGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human CTGF, His TagHuman CTGF, His Tag (Cat. No. CTF-H52H5) ELISA bioactivity

Immobilized Human CTGF, His Tag (Cat. No. CTF-H52H5) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).

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