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Your Position: Home > Immune Checkpoint Proteins > BTLA > Biotinylated Human BTLA (31-150) Protein, His Tag, Avi Tag (Avitag™)

Biotinylated Human BTLA (31-150) Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    BTLA,CD272
  • Source
    Biotinylated Human BTLA (31-150), His Tag, Avi Tag (BTA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Lys 31 - Ser 150 (Accession # AAP44003.1).
    Predicted N-terminus: Lys 31
  • Molecular Characterization
    Online(Lys 31 - Ser 150) AAP44003.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

    The protein has a calculated MW of 17.4 kDa. The protein migrates as 28-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human BTLA (31-150), His Tag, Avi Tag (Cat. No. BTA-H82E6) SDS-PAGE gel

Biotinylated Human BTLA (31-150), His Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human BTLA (31-150), His Tag, Avi TagBiotinylated Human BTLA (31-150), His Tag, Avi Tag (Cat. No. BTA-H82E6) ELISA bioactivity

Immobilized Human HVEM, mouse IgG2a Fc Tag, low endotoxin (Cat. No. HVM-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BTLA (31-150), His Tag, Avi Tag (Cat. No. BTA-H82E6) with a linear range of 0.156-2.5 μg/mL (QC tested).

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