This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 77.0 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
FITC-Labeled Human Siglec-2 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Anti-Human CD22 MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) with a linear range of 0.625-10 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).
293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Siglec-2 / CD22 Protein (Cat. No. SI2-HF2H6, 10 µg/ml) and C. FITC-labeled Protein Control. A. Non-transfected 293 cells and C. FITC-labeled Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Siglec-2 / CD22 Protein (Cat. No. SI2-HF2H6).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $290.00
Price(USD) : $1250.00
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