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Your Position: Home > All Other Proteins > Siglec-2 > FITC-Labeled Human Siglec-2 / CD22 Protein

FITC-Labeled Human Siglec-2 / CD22 Protein

  • Synonym
    CD22, SIGLEC2, BL-CAM, SIGLEC-2, Siglec2, SIGLEC2FLJ22814
  • Source
    FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) is expressed from human HEK293 cells. It contains AA Asp 20 - Arg 687 (Accession # P20273-1). It is the FITC labeled form of Human Siglec-2 (Cat. No. CD2-H52H8).
    Predicted N-terminus: Asp 20
  • Molecular Characterization
    Siglec-2(Asp 20 - Arg 687) P20273-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 77.0 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 1.5-3.5.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) SDS-PAGE gel

FITC-Labeled Human Siglec-2 on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
FITC-Labeled Human Siglec-2FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) ELISA bioactivity

Immobilized Anti-Human CD22 MAb at 10 μg/mL (100 μL/well) can bind FITC-Labeled Human Siglec-2 (Cat. No. SI2-HF2H6) with a linear range of 0.625-10 μg/mL (Ex.488 nm/Em.535 nm) (QC tested).

Evaluation of CAR expression
FACS Analysis of Anti-CD22 CAR Expression
Evaluation of CAR expression

293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human Siglec-2 / CD22 Protein (Cat. No. SI2-HF2H6, 10 µg/ml) and C. FITC-labeled Protein Control. A. Non-transfected 293 cells and C. FITC-labeled Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Siglec-2 / CD22 Protein (Cat. No. SI2-HF2H6).

  • Background
    B-cell receptor CD22 is also known as Sialic acid-binding Ig-like lectin 2 (Siglec-2), B-lymphocyte cell adhesion molecule (BL-CAM), T-cell surface antigen Leu-14, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. CD22 mediates B-cell B-cell interactions, and may be involved in the localization of B-cells in lymphoid tissues. Siglec-2 / CD22 binds sialylated glycoproteins, one of which is CD45. Siglec2 / CD22 plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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