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Your Position: Home > All Other Proteins > Integrin alpha 5 beta 1 > Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His,Avitag&Tag Free

Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, His,Avitag™&Tag Free

  • Synonym
    Integrin alpha 5 beta 1,ITGA5&ITGB1
  • Source
    Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (IT1-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 42 (ITGA5) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1)) P08648-1 (ITGA5) & P05556-1 (ITGB1)

    Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 112.5 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 120-130KDa (ITGA5) and 100-115 kDa (ITGB1) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) SDS-PAGE gel

Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) ELISA bioactivity

Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) with a linear range of 2-78 ng/mL (QC tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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