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>Integrin alpha 5 beta 1
>Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein
Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 112.5 kDa (ITGA5) & 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 120-130KDa (ITGA5) and 100-115 kDa (ITGB1) respectively due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82Wa) with a linear range of 2-78 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $1380.00
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