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Your Position: Home > All Other Proteins > Integrin alpha 5 beta 1 > Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein

Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein

  • Synonym
    Integrin alpha 5 beta 1, ITGA5&ITGB1
  • Source
    Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein (IT1-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 42 (ITGA5) & Gln 21 (ITGB1)
  • Molecular Characterization
    ITGA5&ITGB1(Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1)) P08648-1 (ITGA5) & P05556-1 (ITGB1)

    Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 112.5 kDa (ITGA5) & 83.7 kDa (ITGB1). Subunit ITGA5 is  fused with an acidic tail at the C-terminus and followed by a polyhistidine tag  and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 120-130KDa (ITGA5) and 100-115 kDa (ITGB1) respectively due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82Wa) SDS-PAGE gel

Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human ITGA5 & ITGB1 Heterodimer ProteinBiotinylated Human ITGA5 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82Wa) ELISA bioactivity

Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA5 & ITGB1 Heterodimer Protein (Cat. No. IT1-H82Wa) with a linear range of 2-78 ng/mL (QC tested).

  • Background
    Integrin alpha 5 beta 1 / Integrin α5β1, which is a major cellular receptor for the extracellular matrix protein fibronectin and plays a fundamental role during mammalian development., is composed of α5 (ITGA5/CD49e) and β1 (ITGB1/CD29) subunits. Integrins are adhesion receptors that transmit bidirectional signals across the plasma membrane. The Integrin alpha 5 beta 1 and its primary extracellular matrix ligand fibronectin (Fn) are of great biological importance.
  • References
    • Limited Use License

      The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

      Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Price(USD) : $350.00

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