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Your Position: Home > All Other Proteins > TNFSF11 > Biotinylated Human TNFSF11 / RANKL / CD254 Protein, Fc Tag, Avi Tag

Biotinylated Human TNFSF11 / RANKL / CD254 Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    RANKL,CD254,TRANCE,OPGL,ODF
  • Source
    Biotinylated Human TNFSF11, Fc Tag, Avi Tag (RAL-H82F9) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).
    Predicted N-terminus: Gly
  • Molecular Characterization
    Online(Gly 64 - Asp 245) AAC51762.1

    This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.

    The protein has a calculated MW of 49.2 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Biotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) SDS-PAGE gel

Biotinylated Human TNFSF11, Fc Tag, Avi Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human TNFSF11, Fc Tag, Avi TagBiotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) ELISA bioactivity

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) with a linear range of 0.4-13 ng/mL (QC tested).

Biotinylated Human TNFSF11, Fc Tag, Avi TagBiotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) ELISA bioactivity

Immobilized Human OPG, Fc Tag (Cat. No. TNB-H5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human TNFSF11, Fc Tag, Avi Tag (Cat. No. RAL-H82F9) with a linear range of 0.3-10 ng/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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