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Your Position: Home > Immune Checkpoint Proteins > CD40 Ligand > Biotinylated Human CD40 Ligand / TNFSF5 Protein, His Tag, Avi Tag

Biotinylated Human CD40 Ligand / TNFSF5 Protein, His Tag, Avi Tag (Avitag™)

  • Synonym
    CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
  • Source
    Biotinylated Human CD40 Ligand, His Tag, Avi Tag (CDL-H82Q8) is expressed from human 293 cells (HEK293). It contains AA Glu 108 - Leu 261 (Accession # P29965-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Glu 108 - Leu 261) P29965-1

    This protein carries a polyhistidine tag at the N-terminus and an Avi tag at the C-terminus.

    The protein has a calculated MW of 25.6 kDa. The protein migrates as 28 kDa and 30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD40 Ligand, His Tag, Avi Tag (Cat. No. CDL-H82Q8) SDS-PAGE gel

Biotinylated Human CD40 Ligand, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human CD40 Ligand, His Tag, Avi TagBiotinylated Human CD40 Ligand, His Tag, Avi Tag (Cat. No. CDL-H82Q8) ELISA bioactivity

Immobilized Biotinylated Human CD40 Ligand, His Tag, Avi Tag (Cat. No. CDL-H82Q8) at series of concentration on streptavidin precoated (0.5 μg/well) plate, can bind Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) 2 μg/mL (100 μL/well) with a linear range of 1-40 ng/mL (QC tested).

  • Background
    CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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  • PEGS Boston 2018
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  • Latest Clinical Research Stage:Phase ?

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