Generally usable: Generally usable for removing all forms of DNA and RNA from biological products, reducing viscosity and preventing cell clumping.
Efficient: Efficient reaction, rapid degradation, reduce processing time.
Native: Native structure, tag free, the enzyme activity is more guaranteed.
Ideal: Ideal for DNA and RNA clearance
Ultra pure: Ultra pure: High purity and activity
Specific: Specific nuclease, no protease activity
1. Reagents and solutions preparation
50 mM Tris-HCl, 1 mM MgCl2, pH 8.0 ( * In the case of extensive dilution before use, carrier protein such as 0.1 mg/ml HSA or BSA is generally recommended to avoid any enzyme loss from surface adsorption)
1 mg/ml salmon sperm DNA is dissolved overnight at 4 ℃, in reaction buffer, and is then sonicated on ice to obtain a homogenous solution.
Different dilution of nuclease with reaction buffer.
Trichloroacetic acid (TCA)
2. Standard curve establishment
400 μl substrate + 100 μl enzyme of known activity = 500 μl mixture
Incubate the mixture at 37℃ for 30 min.
Stop the reaction by addition of 400 μl cold TCA and incubate on ice for 10 min.
Centrifuge at 8500g for 5 min.
Measure the absorbance of supernatant at 260 nm.
Lot a standard curve with nuclease of known activities for each set of measurements.
3. Measurement of activity
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of GENIUS™Nuclease is >1.0 x 10e6 unit/mg protein.
Standard curve of nuclease activity for GENIUS™Nuclease.
Notice: We updated the brand name from Benz™Nuclease to GENIUS™Nuclease. The products are the same and the only change is the brand name in the product name and the label.
The purity of GENIUS™Nucleasee was determined by SDS-PAGE reduced and staining overnight with Coomassie Blue.
The purity of GENIUS™Nuclease (Cat. No. BEE-N3116) was more than 95% as determined by SEC-HPLC.
The result of GENIUS™Nuclease activity.
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